Beiträge zur Kenntnis der Flora von Süddalmatien

Ohne Zusammenfassung     

FURTHER CHEMICAL STUDIES ON BLOOD-AQUEOUS HUMOR DYNAMICS

The proportion of SCN, Br, PO₄, urea, levulose, and Fe which remains freely diffusible when added to plasma has been determined by ultrafiltration and "differential" dialysis through a cellophane membrane. After injecting each of these substances as well as Mg and Li into rabbits a continuous record of their concentration in the plasma was obtained for each animal and the concentration in the aqueous humor was also determined and related to the maximum diffusible concentration in the plasma.     

Cellulase Facilitated Squash Preparations of Vicia Faba Chromosomes for Quinacrine Fluorescent Staining

This paper is partially based on work performed under contract with the U. S. Atomic Energy Commission at The University of Rochester Atomic Energy Project and has bcen assigned Report No. UR-3490-238, and has also been partially supported by Grant USPHS GM0933.     

Water solubilization of membrane proteins. Extensive derivatization with a novel polar derivatizing reagent

A reagent and a derivatization procedure have been developed which result in trimesylation of all -OH and -NH2 groups on proteins: serine, threonine, tyrosine, and lysine side chains are all completely derivatized. The parameters affecting the kinetics of trimesylation of serine, threonine, and tyrosine peptides were studied using dinitrophenylated peptides as model systems. Conditions for solubilization of proteins in anhydrous organic solvents for the derivatization are described; removal of blocking groups results in the polar, highly water-soluble protein derivative which behaves as a monomer during gel permeation chromatography in simple aqueous buffers even in the absence of detergents. Synthesis of the tritiated reagent is described, and this reagent was used to monitor the protein derivatization.     

Marathon fatigue: the role of plasma fatty acids, muscle glycogen and blood glucose

The role of carbohydrate depletion in marathon fatigue was examined in 6 marathon runs. Four of the runs were potentially 'fast-time' marathons and culminated in fatigue. The utilization of carbohydrate, lipid and protein, and plasma concentrations of free fatty acids (FFA), glucose and lactate were measured at intervals throughout the runs. The contribution from protein to energy output was low (1-2%). The utilization of lipid was dependent upon plasma concentrations of FFA, which rose throughout the run. The utilization of carbohydrate mirrored that of FFA and thus fell throughout the run. Fatigue was characterized by a drop in running speed, a drop in carbohydrate utilization, an unchanging FFA utilization and a fall in blood glucose. The fall in blood glucose was not seen in the non-fatigued runners. These results are consistent with carbohydrate depletion being the cause of fatigue. The implications of these data are that lipid is the preferred fuel, but is rate-limiting, and that carbohydrate depletion, even though it causes fatigue, ensures an optimal-time marathon.